Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review
Paes Sabrina Moreira, Pupo Yasmine Mendes, Cavenago Bruno Cavalini, Fonseca-Silva Thiago, de Oliveira Santos Carolina Carvalho,
소속 상세정보
( Paes Sabrina Moreira ) - Universidade Federal do Parana Department of Restorative Dentistry
( Pupo Yasmine Mendes ) - Universidade Federal do Parana Department of Restorative Dentistry
( Cavenago Bruno Cavalini ) - Universidade Federal do Parana Department of Restorative Dentistry
( Fonseca-Silva Thiago ) - Universidade Federal dos Vales do Jequitinhonha e Mucuri Department of Dentistry
( de Oliveira Santos Carolina Carvalho ) - Universidade Federal do Parana Department of Restorative Dentistry
Abstract
Objectives: The aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery.
Materials and Methods: This systematic review was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the research question was determined using the population, exposure, comparison, and outcomes strategy. Electronic searches were conducted in the PubMed, Cochrane Library, Science Direct, LILACS, and SciELO databases and in the gray literature (dissertations and thesis databases and Google Scholar) for relevant articles published up to March 2019. Clinical trial studies performed with dental pulp of human permanent or primary teeth, containing concrete information regarding the cryopreservation stages, and with cryopreservation performed for a period of at least 1 week were included in this study.
Results: The search strategy resulted in the retrieval of 185 publications. After the application of the eligibility criteria, 21 articles were selected for a qualitative analysis.
Conclusions: The cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth.
키워드
Cryopreservation; Dental pulp; Mesenchymal stromal cells; Stem cells
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